Associate Scientist– B cell sorting and hybridoma generation


Location: Waltham, Massachusetts

Job Type: Full time


Company Description

For over 20 years, abcam has been providing tools the scientific community needs to enable faster breakthroughs in critical areas like cancer, neurological disorders, infectious diseases, and metabolic disorders.

We believe that to continue making progress, we need to work together in new ways. We need your own unique perspective as well as this of our people to make an always greater impact on the world. This community needs people like you: dedicated, agile and above all audacious so we can truly bring progress forward.

Job Description

About the role

The Associate Scientist will be responsible for RabMAb development by performing lymphocyte isolation, B-cell sorting, and hybridoma generation experiments. Detailed experimental documentation, database entry and report preparation are also required.

Roles & Responsibilities

  • Process tissues including isolation of lymphocytes from spleens, lymph nodes, and blood using Ficoll, and other lymphocyte separation techniques.
  • Operate flow cytometers and cell sorters for single B-cell sorting process.
  • Perform fusion process by PEG fusion and electrofusion machine to generate hybridoma clones in semi-solid media and liquid media.
  • Culture and maintain hybridoma clones to produce antibodies for screening and validation.
  • Culture and maintain rabbit hybridoma fusion partner cell line.
  • Troubleshoot mechanical and software issues related to the flow sorting instrumentation.
  • Work closely with RA scientists and project stakeholders to make project progress smoothly.
  • Update the experimental information on internal database.
  • Manage and regular maintain instruments, equipment, and inventory.
  • Maintain high quality standards within the lab; ensuring the lab is compliant with all external and internal quality standards, and compliance with all health and safety guidelines.


You will have a Master’s degree or Ph.D. in Immunology, Cell Biology, Biochemistry, or a related field. Hand on experiences in mammalian cell culture, multi-parametric flow cytometry/sorting, and be able to do troubleshooting. Experience in hybridoma generation and maintain is preferred. Knowledge of basic immunology concepts. Solid organizational skills including attention to details and multi-tasking skills. Rigorous logical thinking and good communication skills. Active work attitude, strong sense of responsibility.

Additional Information

We know that when it comes to benefits, no one size fits all. Flexibility and choice matter which is why in addition to market competitive salaries, we offer you a flexible benefits package which is tailored to your unique needs and support your financial, physical and emotional wellbeing. This includes our award-winning share scheme, 18 weeks fully paid maternity leave, 6 weeks fully paid paternity leave as well as highly flexible working and much more. Besides, your development will be integral to your experience here. You will grow alongside other talented minds, in ways you may often find unexpected.

When people come together, incredible things happen. Here you’ll work in a safe environment where you can be who you truly are. We’ll champion and celebrate your uniqueness throughout your journey with us. This is how we excel at partnering with the scientific community no matter the challenge, ultimately helping solve the world’s most critical diseases. Find out more about Diversity & Inclusion at abcam.


You’ve got this!